Bioremediation is environment friendly process that utilised a range of communities of. Modeling and experimental investigation fatemeh bahmani 1, seyed ahmad ataei 1 2and mohammad ali mikaili 1department of chemical engineering, shahid bahonar university of kerman, kerman, iran 2department of civil and environmental engineering, amirkabir. Pretreating the soil with biosurfactants produced from bacteria and fungi could improve the success rate. Bioremediation of pahcontaminated soils, sediments, and water can be accomplished in a variety of ways, eg in situ treatment or exsitu methods such as biopiling and composting. Table 1 summarizes the main technologies for cleaning up polluted soils and the estimated costs for each treatment. Bioremediation of crude oil contaminated soil by petroleumdegrading active bacteria jinlan xu school of environmental and municipal engineering, xi an university of architecture and technology, xi an, shaanxi, china 1. The remediation processes can be applied directly to the site of contamination, in situ, or after removing the contaminated soil, ex situ. Cole, compost bioremediation, more than any other soil cleanup technique, results in an enriched soil end product and leaves the earth in better condition than before it was contaminated. Oil drilling can create opportunities for spilled oil and pipeline leaks to occur. The influencing parameters on the bioremediation techniques in the arctic areas section discusses the factors that affect the biodegradation process in hydrocarbon contaminated arctic soils.
Bioremediation of hydrocarbon contaminated soil july 17, 2012 1 technical guidance 14 for contaminated sites operation of soil treatment facilities for the bioremediation of hydrocarbon contaminated soil april 20 1 and. As for all soils, the successful application of bioremediation depends on appropriate biodegradative microbes and environmental conditions in situ. At low hydrocarbon concentrations, the impact on plants is due to the physical impact on soil structure. Bioremediation of hydrocarboncontaminated soil with used mushroom compost. A general essay on bioremediation of contaminated soil. Environmental parameter optimization for bioremediation of petroleum hydrocarbon contaminated soil richard scholzerichard scholze u. Recent research has cast some doubt on this assumption. Bioremediation of a pah contaminated soil was carried out with a bacterial consortium enriched from the soil. Pdf bioremediation proved to be an effective approach to deal with soil contamination, especially in isolated, cold environments such as. Bioremediation and discharge of hydrocarbon impacted soil at. Soil contaminated with petroleum has a serious hazard to human health and causes environmental problems as well.
Soils contaminated with 1 to 2% hydrocarbon can be remediated with the normal salt spill remediation process. Bioremediation and discharge of hydrocarbon impacted soil. The biodegradation rate of bioremediation depends on the soil environment and can be enhanced by microorganism immobilization. Soil contaminated with petroleum hydrocarbons are remediated using a diverse set of physicochemical and biological biodegradation and bioremediation treatment. Bioaugmentation is a promising technology to clean up sites contaminated by the petrochemical industry. Bioremediation of heavy metals and petroleum hydrocarbons in.
Bioremediation of polyaromatic hydrocarbons pahs using. One approach to restoring contaminated soil is to make use of microorganisms able to degrade the toxic compounds in a bioremediation process. Andrea milioni, chemical engineer on contract cooperator university ucbm rome italy 1. Sep 22, 2015 a laboratory study on the bioremediation of diesel contaminated soil with the earthworm eudrilus eugeniae kingberg was conducted. Sutherland td, horne i, lacey mj, harcourt rl, russell. Bioremediation is simpler, less labor intensive and public. Exsitu bioremediation procedure the soil samples were dug to a depth of 015 cm. Bioremediation is a process used to treat contaminated media, including water, soil and subsurface material, by altering environmental conditions to stimulate growth of microorganisms and degrade the target pollutants. Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation fatima m. Bioremediation treatment of hydrocarboncontaminated arctic soils. Mycoremediation of aged petroleum hydrocarbon contaminants in. The excavated top soil, unlike the cow dung and palm kernel husk ash was sun dried for.
Soil bioremediation is attracting interest as a promising and costeffective. The best approach for bioremediation of soil contaminated with diesel oil is the inoculum of microorganisms preselected from their own environment. Bioremediation of petroleum oil contaminated soil and water. Earthwormassisted bioremediation of petroleum hydrocarbon contaminated soil from mechanic workshop ameh a. Nynas uk ab, bitumen refinery, dundee the challenge. Other waste products that have high carbon and nutrient contents can be utilized to compost diesel contaminated soil in a similar manner.
Biological remediation of hydrocarbon and metal contaminated soil offers a better and more environmentally friendly technique that if properly and thoroughly explored can bring our environment into a better place for both plant and animals well being due to its enormous advantages over other treatment methods. Thermally enhanced approaches for bioremediation of hydrocarbon contaminated soils. Theme description the large increase in the past century of industrial development, population growth and urbanization favoured the release of hazardous chemicals in the environment and a. The goal of remediation is to remove, or to make harmless, substances contaminating the soil or groundwater. Evaluation of phytoremediation and bioremediation for. The last type of treatment, treatment 4, is the control. Traditionally soil remediation has been known to be among the most. Army corps of engineers erdccerl, champaign, ilerdccerl, champaign, il jeff salmonjeff salmon fort hood environmental mgmt branchfort hood environmental mgmt branch. Bioremediation of soils contaminated with petroleum solid wastes and. Soil bioremediation approaches for petroleum hydrocarbon polluted. Control involved the use of soil without the addition of bacteria or growing plants to. There is no requirement to characterize the soil as hazardous waste if its managed on the site that it was generated. The investigation revealed total petroleum hydrocarbon tph levels at 75,000 parts per million ppm.
Army corps of engineers erdccerl, champaign, ilerdccerl, champaign, il jeff salmonjeff salmon fort hood environmental mgmt branchfort hood environmental. Physicochemical parameters, heavy metals and total petroleum hydrocarbons were analyzed using aas. Scientific progress in the last two centuries has allowed a great development of industrial production activities, modifying the relationship between mankind and the environment. However, it is known that hydrocarbon biodegradation in soil can be limited by many factors, such as microorganism type, nutrients, ph, temperature, moisture, oxygen, soil properties. For example, soil tillage, which promotes microbial breakdown of pahs, is a cheap but slow option and only works on the top 35 cm of soil. At hydrocarbon concentrations of 4% or greater, some direct toxicity to plants occurs. Us9545652b2 bioremediation of hydrocarboncontaminated. In the following, main techniques for soil remediation will be presented focusing on the cleaning up after hydrocarbon contamination. This project is part of a larger study that seeks to demonstrate the feasibility of bioremediation of petroleum hydrocarbons by naturally present bacteria in contaminated soils in saline conditions and cold climates. Bioremediation of pah contaminated soils in a fluidized bed bioreactor 1 m 3 scale 3 bioremediation of hazardous abandoned armament sites 3. Us9545652b2 bioremediation of hydrocarboncontaminated soil. Bioremediation of heavy metals and petroleum hydrocarbons. In the use of a bioreactor, contaminated soil is mixed with water and nutrients and the mixture is agitated by a mechanical bioreactor to stimulate.
Bioremediation of crude oil contaminated soil using. Working to clean up contaminated land hydrocarbons are one of the most common contaminants that require remediation due to the widespread occurrence of spills and from the regular activities of exploration and production of oil and other petroleumbased products. Bioremediation of hydrocarbon contaminated soil using selected organic wastes author. Petroleum hydrocarbon contamination soil at the seymour johnson air force base near goldsboro, north carolina, is contaminated as.
The soil does not need to be supplemented with additional chitin. Ex situ bioremediation, in which contaminated soil is excavated and treated elsewhere, is an alternative. Introduction bioremediation is a popular approach of cleaning up. The system is partially submerged into the contaminated substrates and stimulates electron transfer by microbial species in order to degrade contaminating hydrocarbons in the substrate, including vadose zone substrates. Growing awareness of the harmful effects of pah pollutants to the environment and human health has led to a marked increase in research into various strategies that might be used to clean up contaminated sites. Soil contamination with petroleum has long been a major environmental problem. In temperate climates there is a sizeable proportion of a soil s biomass that consists of cold adapted bacteria, that can efficiently degrade hydrocarbon at. Bioremediation and biodegradation of hydrocarbon contaminated soils. Two trial plots were established in autumn 2008 to compare two sets of conditions. Article pdf available in international journal of advanced research 46. The application of microorganisms or microbial processes to remove or degrade contaminants from soil is called bioremediation. Bioremediation uses soil microorganisms to degrade polycyclic aromatic hydrocarbons pahs into less toxic compounds and can be performed in situ, without the need for expensive infrastructure or amendments. Us5436160a bioremediation of hydrocarbon contaminated. The paper reports on the bioremediation of a refinery soil containing hydrocarbons in a semi.
Bioremediation of soils contaminated with petroleum solid. The biosurfactant rhamnolipid applied was released by pseudomonas sp. Biowish technology increases the oxidation rate of organic pollutants, making it highly effective to use in the remediation of contaminated soil or polluted bodies of water. Evaluation of phytoremediation and bioremediation for sandy. Pdf bioremediation and biodegradation of hydrocarbon. Pdf bioremediation of hydrocarboncontaminated polar. Bioremediation of petroleum hydrocarbon contaminated soil. A guide for remediation of salt hydrocarbon impacted soil distributed by. Soil samples n84 were collected from different bioremediation treatments. Pdf bioremediation of hydrocarboncontaminated soils in cold. Biological remediation of hydrocarbon and heavy metals.
Contaminated soil samples from an oil storage site with an initial tph concentration of 14,000 mg kg. Pdf on jan 1, 2017, janet olufunmilayo and others published bioremediation of hydrocarboncontaminated soil using organic wastes as. Pdf landfarming is an attractive bioremediation treatment approach for soils contaminated with polycyclic aromatic hydrocarbons pahs. Bioremediation is promising technology for treatment of wide range of contaminants in soil and groundwater, particularly oil contamination bioremediation is sitespecific.
Bioremediation biological remediation techniques have gained wide approval as safe and ecofriendly, but are often insufficient in isolation. Pdf bioremediation of hydrocarboncontaminated soil using. Pdf potentials for biosurfactant enhanced bioremediation. Bioremediation of pah contaminated soils, sediments, and water can be accomplished in a variety of ways, eg in situ treatment or exsitu methods such as biopiling and composting. Bioremediation of hydrocarboncontaminated soil with used.
However, in highly contaminated soils 10 000 mg per kg, the researchers note that pretreating with a more effective thermal or chemical process is currently necessary a drawback of bioremediation. A laboratory study on the bioremediation of diesel contaminated soil with the earthworm eudrilus eugeniae kingberg was conducted. Biosurfactant rhamnolipid was used to increase solubility of the petroleum oil in soil so as to enhance the hydrocarbon bioavailability to the microorganisms. Nynas inherited an old soil treatment bed with their site purchase. Implications of bioremediation of polycyclic aromatic. Bioremediation of hydrocarbon contaminated soil using selected organic wastes. Application of surfactants to contaminated soil and water, at concentrations above their critical micelle. How to evaluate alternative cleanup technologies for.
Bioremediation of hydrocarbon contaminated soil using selected. Bioremediation techniquesclassification based on site of. Bioavailability of hydrophobic organic compounds hocs to microorganisms could be a limiting factor during the biodegradation process. Bioremediation and biodegradation of hydrocarbon contaminated. Bioremediation is increasingly viewed as an appropriate remediation technology for hydrocarboncontaminated polar soils. Bioremediation is the process of utilizing living organisms, microorganisms to degrade pollutants and contaminants from the environment and transform them into less toxic form. In this study, enzymatic analyses and signature lipid biomarkers were employed to evaluate the efficacy of selected bioremediation products on control and oil contaminated soil plots.
Eaob, and other insitu bioremediation methods that rely heavily upon contaminants coming into direct contact with oxidants or reductants present in the subsurface, are often not practical or costeffective in lowpermeability soils such as clays. Vincenzo piemonte, associate professor, university ucbm rome italy 1. Different organisms are employed using various technique of bioremediation according to hydrocarbon present in the contaminated soil. North dakota industrial commission department of mineral resources bismarck, nd 585050840 funded by the oil and gas research council technical author len j. Bioremediation for a soil contaminated with hydrocarbons. Soils, considered suitable for bioremediation, are typically contaminated with hydrocarbons at levels of 0255% by volume of oil concentration huesemann et al. Environmental parameter optimization for bioremediation of. The old soil treatment bed was identified as an environmental liability which required a solution. Bioremediation of hydrocarbon contaminated soils the client.
Earthwormassisted bioremediation of petroleum hydrocarbon. Bioremediation of polycyclic aromatic hydrocarbons. The biological removal of petroleum products using landfarming has been applied commercially in large scale with relative success. The doe supports the use of bioremediation for hydrocarbon contaminated soils where the contaminated soil can be treated to enable it to be used as a resource e. Many methods have been developed for its remediation, including bioremediation, one of the most applied procedures. Comparative bioremediation of soils contaminated with diesel. Week i i festuca idahoensis on potting soil and truck bay test soils 1 untreated control, 2 mycoremediation, 3 bioremediation, and 4 enhanced bacterial from left to right figure f.
The effect of moisture content variation on the bioremediation of hydrocarbon contaminated soils. Bioremediation of hydrocarboncontaminated polar soils. In physicochemical treatment incineration, thermal desorbtion, coker, cement kiln and solvent extraction are used but they have some disadvantages. Biostimulation using organic substances such the poultry manure, cow dung, biochar and food waste are more effective in optimising the process of bioremediation. The process of bioremediation by microorganisms those that break down hydrocarbons can be used to purge the environment of these persistent pollutant particles. Laboratory studies have confirmed that hydrocarbon degrading bacteria typically.
Bioremediation of crude oil contaminated soil by petroleum. Summary of bioremediation techniques for hydrocarbon contaminated soils. The arctic environment is very vulnerable and sensitive to hydrocarbon pollutants. A method for using a bioelectrochemical system for the remediation of contaminated substrates is disclosed. Bioremediation treatment of hydrocarbon contaminated. Low temperature ex situ bioremediation of hydrocarbon. Bioremediation of hydrocarbon pollution can be done by both microorganisms and plans phytoremediation. Not only microorganisms but plants too help in bioremediation phytoremediation. Bioremediation has become an important method for the treatment of terrestrial oil spills and is often favoured over strictly physicalchemical methods.
Us5436160a bioremediation of hydrocarbon contaminated soil. Frankenberger b, a department of soil science, federal university of rio grande do sul, porto alegre rs 91540000, brazil. Evaluation of phytoremediation and bioremediation for sandy soil contaminated with petroleum hydrocarbons. Ex situ bioremediation approaches include use of bioreactors, landfarming, and biopiles. Pilotscale bioremediation of a petroleum hydrocarboncontaminated clayey soil from a subarctic site. Sample verification during the preparation of the tankbottom materials treatment, a composite sample was obtained and sent to a certified laboratory for analytical verification. Bioremediation of hydrocarbon contaminated soil using. Investigations of hydrocarbon spills in farming and biopiles may be useful approaches for polar regions indicate that hydrocarbon degraders, bioremediation of polar soils. The bioremediation of hydrocarbon contaminated soil is enhanced by applying to the soil a hydrocarbon solution of a surfactant selected from the group consisting of. Petroleum pollutants, mainly hydrocarbon, are classified as priority pollutants. For this reason bioremediation is an ments conducted on site in the arctic indicate that land attractive option.
The soil screening guidance is an epa tool for the standardization of the stepbystep evaluation and cleanup of contaminated soils destined to possible residential use of land. Biorem environmental consultants 1601 meadowbrook dr. In many cases, bioremediation is less expensive and more sustainable than other remediation alternatives. A general essay on bioremediation of contaminated soil msu. Soils contaminated with hydrocarbons, regardless of level of hydrocarbon contamination, can be treated at a mines bioremediation facility as long as the contaminated soil was generated on the mine site.